cd4 antibody, anti-mouse, reafinity  (Miltenyi Biotec)

Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: Dose-Response

Article Title: The Effects of Tibetan Medicine Renqing Changjue Extracts on Cyclophosphamide-Induced Immunosuppression in a Mouse Model

doi: 10.1177/15593258261454535

Figure Lengend Snippet: The effect of RQCJ on the proportion of CD4 + T and CD8 + T cells. (A) CD4 + /CD8 + T ratio in the different groups; (B-F) The flow cytometric analysis of CD4 + T and CD8 + T cells. Compared with the control group: ## p < 0.01. Compared with the CTX group: * p < 0.05, * * p < 0.01. (n = 12, one-way ANOVA was used for data analysis).

Article Snippet: We transferred 100 μL cell suspensions into flow cytometry tubes and added 10 μL of anti-mouse CD4 + T antibodies (FITC anti-mouse CD4, Clone: RM4-5, Concentration: 0.5 mg/mL) and CD8 + T antibodies (PE anti-mouse CD8a, Clone: 53-6.7, Concentration: 0.2 mg/mL) labeled with different fluorochromes (Dakewe, China).

Techniques: Control

Journal: mAbs

Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance

doi: 10.1080/19420862.2026.2674236

Figure Lengend Snippet: T-cell activation and antitumor activity of surrogate 1 + 1 and 2 + 1 H10 TCEs. a) Binding of H10-sTCE1 + 1 and H10-sTCE2 + 1 to isolated mouse splenocytes. b) Activation of CD4+ and CD8+ splenocytes by TCEs in co-culture with CT-26 EGFRwt cells. c) Tumor growth inhibition mediated by different doses of H10-sTCE1 + 1 and H10-sTCE2 + 1 in a CT-26 EGFRwt syngeneic model in mice. d) Comparison of tumor volumes at day 17.

Article Snippet: For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461).

Techniques: Activation Assay, Activity Assay, Binding Assay, Isolation, Co-Culture Assay, Inhibition, Comparison

Journal: mAbs

Article Title: Novel bispecific T-cell engagers overcoming acquired EGFR resistance

doi: 10.1080/19420862.2026.2674236

Figure Lengend Snippet: T-cell activation and antitumor activity of surrogate 1 + 1 and 2 + 1 H10 TCEs. a) Binding of H10-sTCE1 + 1 and H10-sTCE2 + 1 to isolated mouse splenocytes. b) Activation of CD4+ and CD8+ splenocytes by TCEs in co-culture with CT-26 EGFRwt cells. c) Tumor growth inhibition mediated by different doses of H10-sTCE1 + 1 and H10-sTCE2 + 1 in a CT-26 EGFRwt syngeneic model in mice. d) Comparison of tumor volumes at day 17.

Article Snippet: For analysis of early T-cell activation for co-cultures of CT-26 cells expressing human EGFRwt and splenocytes isolated from BALB/c mice, T cells were stained with antibodies directed against mouse CD3 (Miltenyi Biotec #130–119-758), CD4 (Miltenyi Biotec #130–118-568), CD8 (Miltenyi Biotec #130–123-781) and CD69 (Miltenyi Biotec #130–115-461).

Techniques: Activation Assay, Activity Assay, Binding Assay, Isolation, Co-Culture Assay, Inhibition, Comparison